Novel Antimicrobial Polyelectrolytes Nanofilm Coatings Using the Layer By Layer Technique
Mazen Khaled1, *, Basel Abu-Sharkh2, Amjad Khalil3, Abdallah Manda1, Esam Amr2
Identifiers and Pagination:Year: 2008
First Page: 1
Last Page: 4
Publisher Id: TONMJ-1-1
Article History:Received Date: 26/08/2008
Revision Received Date: 08/10/2008
Acceptance Date: 09/10/2008
Electronic publication date: 26/11/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A novel method using the layer by layer (LBL) technique was investigated to deposit polyelectrolytes with antibacterial properties. A glass substrate was coated by a cationic biguanide followed by the anionic polystyrene sulfonate until a total of twenty layers were deposited. The layers thickness was measured by ellipsometry and the surface morphology was scanned by an atomic force microscope. The layers thickness reached 60nm. The coated and uncoated glass was immersed into tubes containing a nutrient broth media inoculated with Proteus sp., (a gram negative , rod, glucose fermenting bacteria) in a concentration of (~105 cells/mL) and incubated at 37°C for 24 hours. The SEM (Scanning Electron Microscope) micrographs showed a significant reduction in the settlement of Proteus sp. Colonies on the glass coated with polyelectrolytes.